Growth Hormone 1 抗体
(1 validation)Quick Overview for Growth Hormone 1 抗体 (ABIN235517)
抗原
See all Growth Hormone 1 (GH1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 （详细）
- Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %
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产品特性
- MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)
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纯化方法
- Protein A Chromatography. Ascites
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过滤
- 0.2 μm filtered
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免疫原
- hGH isolated from human pituitary gland
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亚型
- IgG1
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anti-Growth Hormone 1 (GH1) antibody
GH1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GH-3155 unconjugated
anti-Growth Hormone 1 (GH1) (AA 28-217) antibodyGH1 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Hormone 1 (GH1) (AA 58-187) antibodyGH1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GH-1371 unconjugated
anti-Growth Hormone 1 (GH1) (AA 1-217) antibodyGH1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3H1C2 unconjugated
anti-Growth Hormone 1 (GH1) (AA 28-216) antibodyGH1 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Hormone 1 (GH1) (AA 1-217) antibodyGH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8G6 unconjugated
anti-Growth Hormone 1 (GH1) antibodyGH1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Growth Hormone 1 (GH1) (AA 28-216) antibodyGH1 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Hormone 1 (GH1) (AA 27-216) antibodyGH1 适用: 犬 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Hormone 1 (GH1) (AA 1-217) antibodyGH1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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限制
- 仅限研究用
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- by
- NanoTemper Technologies
- No.
- #102719
- 日期
- 2018.11.05
- 抗原
- GH1
- Lot Number
- H0833.1
- Method validated
- Unfolding Profile
- Positive Control
- ABIN235517
- Negative Control
- Notes
Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a Ti at 76.3°C. This suggests that the protein is properly folded and functional.
- Primary Antibody
- ABIN235517
- Secondary Antibody
- Full Protocol
- Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #102719 (Unfolding Profile)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Unfolding profile of ABIN235517. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 76.3°C.]()
Unfolding profile of ABIN235517. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 76.3°C.
Full Methods -
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状态
- Liquid
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缓冲液
- 10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. -
储存条件
- 4 °C/-80 °C
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储存方法
- Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.
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有效期
- 1 week
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- Growth Hormone 1 (GH1)
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别名
- Growth Hormone (hGH)
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途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
抗原
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