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Growth Hormone 1 抗体

This anti-Growth Hormone 1 antibody is a 小鼠 单克隆 antibody detecting Growth Hormone 1 in ELISA. Suitable for 人. Independently validated for use in Unfolding Profile.
产品编号 ABIN235517
发货至: 中国

Quick Overview for Growth Hormone 1 抗体 (ABIN235517)

抗原

See all Growth Hormone 1 (GH1) 抗体
Growth Hormone 1 (GH1)

适用

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宿主

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小鼠

克隆类型

  • 98
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单克隆

标记

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This Growth Hormone 1 antibody is un-conjugated

应用范围

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ELISA

克隆位点

090-10154
  • 交叉反应 (详细)

    Cross-reactivity: Synthetic hGH (Somatonorm) 100 % Human Placental Lactogen <0.00 % Human Prolactin <0.00 %

    产品特性

    MAb to hGH, Monoclonal antibody to Human Growth Hormone (hGH)

    纯化方法

    Protein A Chromatography. Ascites

    过滤

    0.2 μm filtered

    免疫原

    hGH isolated from human pituitary gland

    亚型

    IgG1
  • 应用备注

    Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

    限制

    仅限研究用
  • 生效 #102719 (Unfolding Profile)
    '独立验证'标志
    by
    NanoTemper Technologies
    No.
    #102719
    日期
    2018.11.05
    抗原
    GH1
    Lot Number
    H0833.1
    Method validated
    Unfolding Profile
    Positive Control
    ABIN235517
    Negative Control
    Notes

    Passed. ABIN235517 showed a clear unfolding profile with one detected unfolding event with a Ti at 76.3°C. This suggests that the protein is properly folded and functional.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN235517
    Secondary Antibody
    Full Protocol
    • Dilute ABIN235517 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.33µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Experimental Notes

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • 状态

    Liquid

    缓冲液

    10 mM Phosphate, pH 7.4 containing 150 mM Sodium chloride, 0.1 % Sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles.
    Centrifuge product if not completely clear after standing at room temperature.
    Prepare working dilution only prior to immediate use.

    储存条件

    4 °C/-80 °C

    储存方法

    Short term (up to 7 days) store at 2-8 °C. Long term, aliquot and store at <-48 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

    有效期

    1 week
  • 抗原

    Growth Hormone 1 (GH1)

    别名

    Growth Hormone (hGH)

    途径

    NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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