EGFR 抗体 (pSer1047)
Quick Overview for EGFR 抗体 (pSer1047) (ABIN235074)
抗原
See all EGFR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer1047
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交叉反应 （详细）
- The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. Clone 1H9 specifically recognizes EGFR phosphorylated at Serine 1047. Reacts with Human, mouse and dog.
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产品特性
- Cell lysate of 2 x 10^6 vanadate treated HepG2 cells.
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纯化方法
- Thiophilic adsorption and size exclusion Chromatography. Cell culture
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免疫原
- Synthetic phosphopeptide corresponding to AA residues surrounding Serine 1047
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亚型
- IgG1
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR 适用: 人, 小鼠, 大鼠, Pig, 猴, Cow, 小鸡, 犬, 马, 兔, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibodyEGFR 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR 适用: 人, 大鼠 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. EGFR Transactivation Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20.000 cell per lane). The blot was probed with 0.5- g/mL K67190M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: Forskolin Lane 7: Lonomycin Lane 4: LPA Lane 8: Taxol
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 1 mL water for 15 minutes at room temperature.
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缓冲液
- Lyophilized from PBS, PEG, and Sucrose, 0.09 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原
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