CDK2 抗体 (pThr160)
Quick Overview for CDK2 抗体 (pThr160) (ABIN232740)
抗原
See all CDK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr160
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特异性
- Detects cdk2 only when phosphorylated at threonine 160. Weakly cross-reacts with cdc2 phosphorylated at Thr161. Does not cross-react with other phosphorylated cyclin-dependent kinases. Species cross-reactivity: Human, mouse and rat.
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纯化方法
- Protein A purified
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免疫原
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Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2 (KLH coupled).
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IP (1:150), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5 % BSA, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:150. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
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别名
- CDK2
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背景
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Name/Gene ID: CDK2
Subfamily: CDC2/CDK
Family: Protein Kinase
Synonyms: CDK2, Cell division protein kinase 2, CDC2-related protein kinase, p33 protein kinase, p33(CDK2), CDKN2, Cyclin-dependent kinase 2 -
基因ID
- 1017
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UniProt
- P24941
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
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