Crk 抗体 (pTyr221)
Quick Overview for Crk 抗体 (pTyr221) (ABIN229989)
抗原
See all Crk (CRK) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr221
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特异性
- Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Cross-reactivity: Tyr207 phosphorylated CrkL. Does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human and hamster. Species sequence homology: mouse and rat 100 % .
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr221 of human CrkII.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, WB (1:1000)
Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000. Note: for Western Blots incubate membrane with diluted antibody in 5 % BSA, 1X TBS, and 0.1 % Tween-20 at 4?C with gentle shaking overnight. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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- Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
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别名
- CRK
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背景
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Name/Gene ID: CRK
Synonyms: CRK, Adapter molecule crk, CRKII, Proto-oncogene c-Crk, p38 -
基因ID
- 1398
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UniProt
- P46108
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途径
- Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
抗原
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