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14-3-3 sigma/SFN 抗体

This 小鼠 单克隆 antibody specifically detects 14-3-3 sigma/SFN in WB, IHC, IF, IHC (p) 和 IP. It exhibits reactivity toward 人.
产品编号 ABIN229459
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for 14-3-3 sigma/SFN 抗体 (ABIN229459)

抗原

See all 14-3-3 sigma/SFN (SFN) 抗体
14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This 14-3-3 sigma/SFN antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • 特异性

    Recognizes human 14-3-3 sigma protein (26-30kD). Does not cross-react with other isoforms of 14-3-3.

    纯化方法

    Protein G purified

    免疫原

    Recombinant human 14-3-3 sigma protein (26-30kD). Cellular Localization: Cytoplasmic.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: IF, IHC, IHC-P, IP, WB (1 μg/mL)

    Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate. Use Protein G. Western Blot: 1 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: HT29 cells and skin.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Supplied as a liquid in PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

    别名

    SFN / Stratifin / 14-3-3 Sigma

    背景

    Name/Gene ID: SFN

    Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS

    基因ID

    2810

    UniProt

    P31947

    途径

    p53 Pathway, Myometrial Relaxation and Contraction
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