14-3-3 sigma/SFN 抗体
Quick Overview for 14-3-3 sigma/SFN 抗体 (ABIN229459)
抗原
See all 14-3-3 sigma/SFN (SFN) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human 14-3-3 sigma protein (26-30kD). Does not cross-react with other isoforms of 14-3-3.
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纯化方法
- Protein G purified
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免疫原
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Recombinant human 14-3-3 sigma protein (26-30kD). Cellular Localization: Cytoplasmic.
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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应用备注
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Approved: IF, IHC, IHC-P, IP, WB (1 μg/mL)
Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 2 μg/mg protein lysate. Use Protein G. Western Blot: 1 μg/mL for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 2-4 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: HT29 cells and skin. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Supplied as a liquid in PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- SFN / Stratifin / 14-3-3 Sigma
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背景
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Name/Gene ID: SFN
Synonyms: SFN, 14-3-3 sigma, 14-3-3 protein sigma, HME1, Stratifin, YWHAS -
基因ID
- 2810
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UniProt
- P31947
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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