Insulin Receptor 抗体 (Exon 7)
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Quick Overview for Insulin Receptor 抗体 (Exon 7) (ABIN228599)
抗原
See all Insulin Receptor (INSR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 469-592, Exon 7
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特异性
- Recognizes human Insulin receptor, alpha subunit. Inhibits insulin binding (~80 % ) and mimics insulin action. Weakly reacts with bovine, porcine and sheep. Does not react with rabbit, mouse and rat.
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纯化方法
- Protein A purified
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免疫原
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IM-9 lymphocytes followed by purified insulin receptor. MW of Antigen: 135kD. Epitope: aa469-592 (exon 7/8). Cellular Localization: Cell membrane.
Type of Immunogen: Cells -
亚型
- IgG2a kappa
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应用备注
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Approved: ELISA, Func, IP
Usage: Suitable for use in ELISA, Immunoprecipitation, Tyrosine Kinase Assay and Inhibition of insulin binding. May also be used as insulin-like agonist. ELISA: Both as capture and detection. Immunoprecipitation (Use Protein G): 2 μg/mg protein lysate. Tyrosine Kinase Assay: Antibody-mediated capture on microtiter plates. Positive control: IM-9 lymphocyte cells and Placenta. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM PBS, pH 7.4. Glycerol free.
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储存液
- Azide free
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Insulin Receptor (INSR)
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别名
- INSR / Insulin Receptor
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背景
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Name/Gene ID: INSR
Subfamily: Insulin receptor
Family: Protein Kinase
Synonyms: INSR, CD220, ILP receptor, Insulin receptor, IR, HHF5, CD220 antigen -
基因ID
- 3643
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UniProt
- P06213
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途径
- NF-kappaB Signaling, RTK signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
抗原
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