Phospholipase C gamma 1 抗体
Quick Overview for Phospholipase C gamma 1 抗体 (ABIN226218)
抗原
See all Phospholipase C gamma 1 (PLCG1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
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纯化方法
- Protein G purified
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免疫原
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Purified soluble phospholipase C (PLC) g-1 from bovine brain.
Type of Immunogen: Purified protein -
亚型
- IgG
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应用备注
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Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 μg Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 μg/mL detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- dH2O, 40-50 % glycerol
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
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别名
- PLCG1
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背景
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Name/Gene ID: PLCG1
Family: Phospholipase
Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1 -
基因ID
- 5335
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UniProt
- P19174
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途径
- RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
抗原
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