PKC alpha 抗体 (pSer657, Tyr658)
Quick Overview for PKC alpha 抗体 (pSer657, Tyr658) (ABIN222045)
抗原
See all PKC alpha (PKCa) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer657, Tyr658
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特异性
- Detects an 82kD protein corresponding to the molecular mass of phosphorylated PKCa on SDS-PAGE immunoblots of neonatal rat brain lysate. Similar results were seen in other PKCa-expressing cells, such as human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb, but not other PKC isoforms in rat brain lysate.
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纯化方法
- Immunoaffinity purified
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免疫原
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Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to a dual phosphorylation site found in PKCbI (Ser-661/Tyr-662). These human sequences are highly conserved in rat and mouse PKCa and PKCbI. In addition, this dual site is homologous to threonine 674 and tyrosine 675 in human PKCg, although this reactivity has not been confirmed.
Type of Immunogen: Synthetic peptide
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anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Protein Kinase C, alpha (PKCa) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, IHC, IP, IF, FACS, ICC 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibodyPKCa 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (pThr638) antibodyPKCa 适用: 人, 小鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated
anti-Protein Kinase C, alpha (PKCa) (pThr638) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibodyPKCa 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB01012 unconjugated
anti-Protein Kinase C, alpha (PKCa) (C-Term) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) antibodyPKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (N-Term) antibodyPKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (AA 75-103), (N-Term) antibodyPKCa 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB20769 unconjugated
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应用备注
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Approved: ELISA, WB
Usage: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 50 % glycerol, 1 mg/mL BSA, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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别名
- PRKCA / PKC-Alpha
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背景
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Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase
Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha -
基因ID
- 5578
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UniProt
- P17252
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途径
- WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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