MTOR 抗体 (Ser2481)
Quick Overview for MTOR 抗体 (Ser2481) (ABIN220569)
抗原
See all MTOR (mTOR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser2481
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特异性
- Detects endogenous levels of total human mTOR. It cross-reacts weakly with some other proteins based on Western analysis. Species cross-reactivity: Mouse, monkey and rat.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser2481 of human mTOR.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IP (1:100), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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别名
- MTOR
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背景
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Name/Gene ID: MTOR
Subfamily: PI3/PI4
Family: Protein Kinase
Synonyms: MTOR, FRAP1, FRAP2, FRAP, Mammalian target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, RAPT1, Rapamycin target protein 1 -
基因ID
- 2475
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UniProt
- P42345
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途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
抗原
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