HSP27 抗体 (pSer82)
Quick Overview for HSP27 抗体 (pSer82) (ABIN220305)
抗原
See all HSP27 (HSPB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer82
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特异性
- Detects endogenous human HSP27 only when phosphorylated at serine 82. Does not recognize other heat shock proteins. Species cross-reactivity: Monkey.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser82 of human HSP27.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ICC, IHC, IHC-P, WB (1:1000)
Usage: Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry (ABC). Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1XTBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:500. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSPB1 / HSP27
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背景
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Name/Gene ID: HSPB1
Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27 kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067 -
基因ID
- 3315
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原
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