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ErbB2/Her2 抗体 (Cytoplasmic Domain)

This 小鼠 单克隆 antibody specifically detects ErbB2/Her2 in WB, IHC 和 IP. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN219136
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for ErbB2/Her2 抗体 (Cytoplasmic Domain) (ABIN219136)

抗原

See all ErbB2/Her2 抗体
ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This ErbB2/Her2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
  • 抗原表位

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    Cytoplasmic Domain

    特异性

    Recognizes human c-erbB-2 (HER-2, neu). Species cross-reactivity: Mouse and rat.

    纯化方法

    Protein G purified

    免疫原

    Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein. MW of Antigen: 185kD. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane.

    亚型

    IgG1
  • 应用备注

    Approved: IHC (2 - 4 μg/mL), IP, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 μg/mg protein lysate. Western Blot: 1-2 μg/mL for 2hr at RT. Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

    别名

    ERBB2 / HER2

    背景

    Name/Gene ID: ERBB2
    Subfamily: EGF Receptor
    Family: Protein Kinase

    Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1

    基因ID

    2064

    UniProt

    P04626

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
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