ErbB2/Her2 抗体 (Cytoplasmic Domain)
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Quick Overview for ErbB2/Her2 抗体 (Cytoplasmic Domain) (ABIN219136)
抗原
See all ErbB2/Her2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cytoplasmic Domain
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特异性
- Recognizes human c-erbB-2 (HER-2, neu). Species cross-reactivity: Mouse and rat.
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纯化方法
- Protein G purified
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免疫原
- Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein. MW of Antigen: 185kD. Epitope: Cytoplasmic domain. Cellular Localization: Cell membrane.
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亚型
- IgG1
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应用备注
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Approved: IHC (2 - 4 μg/mL), IP, WB (1 - 2 μg/mL)
Usage: Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and Denatured) (Use Protein G): 2 μg/mg protein lysate. Western Blot: 1-2 μg/mL for 2hr at RT. Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: SKBR-3 or T47D cells, or breast carcinoma. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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别名
- ERBB2 / HER2
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背景
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Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 -
基因ID
- 2064
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UniProt
- P04626
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
抗原
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