EGFR 抗体 (AA 1166-1180)
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Quick Overview for EGFR 抗体 (AA 1166-1180) (ABIN218581)
抗原
See all EGFR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1166-1180
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特异性
- Peptide synthesized using conventional technology. The sequence of the epitope is: NH2-C-S-L-D-N-P-D-Y-Q-Q-D-F-F-P-K-E-COOH and corresponds to amino acids 1166-1180 of the EGFR molecule. This maps to a region near the carboxy-terminus which is identical in human, mouse and rat EGFR. The amino terminal cysteine was synthesized to facilitate carrier coupling.
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纯化方法
- Sterile filtered
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免疫原
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Type of Immunogen: Native protein
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应用备注
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Approved: ELISA (1:10000 - 1:50000), IHC, IHC-P (1:500), IP, WB (1:1000 - 1:10000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:500. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Serum.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
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Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1 -
基因ID
- 1956
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原
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