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GPR84 抗体 (AA 21-120)

Name: GPR84 抗体 (AA 21-120)
SKU: ABIN2173922
Availability: OutOfStock

The 兔多克隆 anti-GPR84 antibody is suitable to detect GPR84 in samples from 人, 小鼠和兔. It has been validated for WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p) 和 IHC (fro).

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (GPR84 抗体 (AA 21-120))

FACS

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 抗体 (AA 21-120))

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 抗体 (AA 21-120))

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (GPR84 抗体 (AA 21-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (GPR84 抗体 (AA 21-120))

IHC (p)

5 images

产品编号 ABIN2173922

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GPR84 抗体 (AA 21-120) (ABIN2173922)

抗原

See all GPR84 抗体

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

适用

人, 小鼠, 兔

宿主

兔

克隆类型

多克隆

标记

This GPR84 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
16

7

5

3

3

2

2

2

1

1

1

1

1

1

1

1
AA 21-120

交叉反应

人, 小鼠, 兔

预测反应

Cow,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

亚型

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 166-180), (Extracellular) antibody
GPR84 适用: 人 WB, FACS, IHC, LCI, IF 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 适用: 人, 马, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 166-180), (Extracellular) antibody (PE)
GPR84 适用: 人 FACS, LCI 宿主: 兔 Polyclonal PE

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 适用: 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

别名

GPCR EX33

背景

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

基因ID

53831

UniProt

Q9NQS5