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GPR84 抗体 (AA 21-120)

Name: GPR84 抗体 (AA 21-120)
SKU: ABIN2173922
Availability: OutOfStock

This anti-GPR84 antibody is a 兔多克隆 antibody detecting GPR84 in WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和兔.

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 抗体 (AA 21-120))

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 抗体 (AA 21-120))

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (GPR84 抗体 (AA 21-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (GPR84 抗体 (AA 21-120))

IHC (p)

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (GPR84 抗体 (AA 21-120))

FACS

5 images

产品编号 ABIN2173922

发货至: 中国

Quick Overview for GPR84 抗体 (AA 21-120) (ABIN2173922)

抗原

See all GPR84 抗体

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

适用

人, 小鼠, 兔

宿主

兔

克隆类型

多克隆

标记

This GPR84 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
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AA 21-120

交叉反应

人, 小鼠, 兔

预测反应

Cow,Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

亚型

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 166-180), (Extracellular) antibody
GPR84 适用: 人 WB, FACS, IHC, LCI, IF 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 适用: 人, 马, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 适用: 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) antibody
GPR84 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

别名

GPCR EX33

背景

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

基因ID

53831

UniProt

Q9NQS5