CDK2 抗体 (AA 201-298) (Biotin)
Quick Overview for CDK2 抗体 (AA 201-298) (Biotin) (ABIN2172817)
抗原
See all CDK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 201-298
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交叉反应
- 人, 小鼠
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预测反应
- Rat,Cow,Pig,Horse,Rabbit
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human CDK2
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亚型
- IgG
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anti-Cyclin-Dependent Kinase 2 (CDK2) antibody
CDK2 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 211-298) antibodyCDK2 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) antibodyCDK2 适用: 人, 猴 WB, FACS, IF 宿主: 小鼠 Monoclonal 4B4 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 197-295) antibodyCDK2 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal 1A6 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) antibodyCDK2 适用: 人 WB, IHC, ELISA, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) antibodyCDK2 适用: 人, 猴 WB, FACS, IF 宿主: 小鼠 Monoclonal 1C4 unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 81-298) antibodyCDK2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 94-198) antibodyCDK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 93-211) antibodyCDK2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 2 (CDK2) (pThr160) antibodyCDK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C for 12 months.
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有效期
- 12 months
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- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
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别名
- CDK2
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背景
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Synonyms: CDKN2, p33(CDK2), Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2
Background: Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis, regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication.
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基因ID
- 1017
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UniProt
- P24941
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
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