beta Actin 抗体 (N-Term)
Quick Overview for beta Actin 抗体 (N-Term) (ABIN215582)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Recognizes human beta Actin. Species cross-reactivity: mouse, rat, monkey. Expected to cross-react with a wide range of species due to sequence homology.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide from the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ICC, IF (1:50), IP, WB (1:500 - 1:2500)
Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control. Immunofluorescence: 1:50. Positive control: HeLa whole cell lysate and 3T3 cells (mouse). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-citrate/phosphate buffer, pH 7-8, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- ACTB / Beta Actin
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背景
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Name/Gene ID: ACTB
Synonyms: ACTB, Actin, cytoplasmic 1, Beta-actin, Beta actin, BRWS1, Actin, beta, Beta cytoskeletal actin, PS1TP5-binding protein 1, PS1TP5BP1 -
基因ID
- 60
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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