p21 抗体 (AA 58-77)
Quick Overview for p21 抗体 (AA 58-77) (ABIN215393)
抗原
See all p21 (CDKN1A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 58-77
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特异性
- Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
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纯化方法
- Protein A purified
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免疫原
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Full length recombinant human p21Cip1/WAF1.
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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应用备注
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Approved: Flo (2 μg/mL), IF (1 - 5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB
Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 μg/mL. Flow Cytometry: 2 μg/mL. Western Blot (chemiluminescence): 0.1-1 μg/mL. Immunohistochemistry (frozen/paraffin): 5 μg/mL. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 μg/IP reaction. Immunoprecipitates p21 but not associated proteins. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Supplied s a liquid in PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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别名
- CDKN1A / WAF1 / p21
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背景
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Name/Gene ID: CDKN1A
Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21 -
基因ID
- 1026
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UniProt
- P38936
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途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
抗原
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