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HSP27 抗体 (Biotin)

This anti-HSP27 antibody is a 小鼠 单克隆 antibody detecting HSP27 in WB, IHC, ELISA, IP 和 ICC. Suitable for 人 和 猴.
产品编号 ABIN215210
发货至: 中国

Quick Overview for HSP27 抗体 (Biotin) (ABIN215210)

抗原

See all HSP27 (HSPB1) 抗体
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

适用

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人, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This HSP27 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 特异性

    Recognizes a ~27kD protein, corresponding to the apparent molecular mass of HSP27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). Does not exhibit reactivity with rodent HSP25, alphaA, alphaB or beta-crystallin proteins.

    纯化方法

    Protein G purified

    免疫原

    Purified HSP24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, HSP27.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: ELISA, ICC (10 μg/mL), IHC (5 μg/mL), IP, WB (1 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunoelectron Microscopy, Apoptosis Induction, and EIA/ELISA. Western Blot (ECL): 1 μg/mL. Immunohistochemistry: 5 μg/mL. immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: H1838-01: HeLa Heat Shocked Cell Lysate. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    别名

    HSPB1 / HSP27

    背景

    Name/Gene ID: HSPB1

    Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

    基因ID

    3315

    UniProt

    P04792

    途径

    MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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