HSP27 抗体
Quick Overview for HSP27 抗体 (ABIN215209)
抗原
See all HSP27 (HSPB1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.
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纯化方法
- Protein G purified
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免疫原
- Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.
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亚型
- IgG1
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应用备注
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Approved: ELISA, ICC (10 μg/mL), IF, IHC (5 μg/mL), IP (12.5 μg/mL), WB
Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. Western: Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL. Immunohistochemistry: 5 μg/mL. Immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 mM PMSF, 50 % glycerol.
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储存液
- PMSF
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSPB1 / HSP27
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背景
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Name/Gene ID: HSPB1
Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27 kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067 -
基因ID
- 3315
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原
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