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CD44 抗体 (PE)

Name: CD44 抗体 (PE)
Brand: antibodies-online
SKU: ABIN2144883
Availability: OutOfStock

CD44 适用: 人 FACS, IHC (p), IP 宿主: 小鼠 Monoclonal 4AP2A1 PE

FACS

1 image

产品编号 ABIN2144883

发货至: 中国

Datasheet as PDF

抗原 See all CD44 抗体

CD44
适用
281

137

71

31

25

23

14

13

10

9

8

7

7

5

5

4

2

2

1

1

1

1

1

1
人
宿主
165

120

74

1

1
小鼠
Compatible Secondaries
克隆类型
248

111

2
单克隆
标记
165

41

29

22

16

7

6

5

5

5

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD44 antibody is conjugated to PE
应用范围
264

148

86

71

69

65

55

41

40

30

26

15

7

6

5

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

产品特性

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1(pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells.CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells and the extracellular matrix.

克隆位点

4AP2A1

亚型

IgG2a

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应用备注

Optimal working dilution should be determined by the investigator.

实验流程
- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
限制

仅限研究用
状态

Liquid

缓冲液

Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C
抗原

CD44

别名

CD44 (CD44 产品)

途径

Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling