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- 抗原 See all CD35 (CR1) 抗体
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD35 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 产品特性
- 4AE11 reacts with CR1 , the receptor for the complement component C3b /C4, composed of four different allotypes (160, 190, 220 and 150 kDa). CD35 antigen is expressed on erythrocytes, neutrophils, monocytes, B -lymphocytes and 10-15 % of T -lymphocytes. CD35 is catagorized as a regulator of complement avtivation. It binds complement components C3b and C4b, mediating phagocytosis by granulocytes and monocytes . Application: Removal and reduction of excessive amounts of complement fixing immune complexes in SLE and other auto-immune disorder.This clone is cross reactive with non-human primate.
- 克隆位点
- 4AE11
- 亚型
- IgG1
- Top Product
- Discover our top product CR1 Primary Antibody
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anti-Complement Component Receptor 1 (CD35) (CR1) (AA 1780-2039) antibody
CR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 420-550) antibodyCR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibody (FITC)CR1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal E11 FITC
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 901-1095) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 9A9 unconjugated Recombinant Antibody
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 FACS, DNA Mic 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Baboon, Cynomolgus, 猕猴 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal E11 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
- 别名
- CD35 (CR1 产品)
- 别名
- C3BR antibody, C4BR antibody, CD35 antibody, KN antibody, C3DR antibody, CD21 antibody, Cr-1 antibody, Cr-2 antibody, Cr1 antibody, CR1-L antibody, CR2 antibody, CR1 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, complement receptor 2 antibody, complement component (3d/Epstein Barr virus) receptor 2 antibody, complement receptor type 1 antibody, CR1 antibody, Cr2 antibody, CR2 antibody, LOC718120 antibody
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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