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- 抗原 See all CD40 抗体
- CD40
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD40 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4A3A8 reacts with CD40 (Bp50 ), a member of the TNF receptor family with 48 kDa MW. which is expressed on B lymphocytes including pro-B through to plasma cells but not on monocytes nor granulocytes. CD40 also expressed on dendritic cells and CD34+ hemopoietic cell progenitor. CD40 Molecule involved in regulation of B-cell growth, differentiation and Isotype-switching of Ig and up-regulates adhesion molecules on dendritic cells as well as promotes cytokine production in macrophages and dendritic cells. CD40 antibodies has been reported to co-stimulate B-cell proleferation with anti-m or phorbol esters. It may be an important target for control of graft rejection, T cells and antibody- mediated autoimmune diseases.
- 克隆位点
- 4A3A8
- 亚型
- IgG2b
- Top Product
- Discover our top product CD40 Primary Antibody
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anti-CD40 (CD40) (AA 26-187) antibody
CD40 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IF, StM, DNA Mic 宿主: 小鼠 Monoclonal C40-1605 unconjugated
anti-CD40 (CD40) (AA 29-107) antibodyCD40 适用: 人 FACS, ELISA, IHC, StM, Coat 宿主: 小鼠 Monoclonal C40-2383 unconjugated
anti-CD40 (CD40) (C-Term) antibodyCD40 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) (AA 21-193) antibodyCD40 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4C3B8 unconjugated
anti-CD40 (CD40) (Extracellular Domain) antibodyCD40 适用: 人 FACS, IHC, St 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-CD40 (CD40) (AA 1-360) antibodyCD40 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IP, Act 宿主: 兔 Chimeric G28-5 unconjugated Recombinant Antibody
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a unconjugated
anti-CD40 (CD40) antibody (FITC)CD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a FITC
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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