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E2F1 抗体 (pSer364)

This 兔 多克隆 antibody specifically detects E2F1 in WB, ELISA, IHC 和 IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN214450
发货至: 中国
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Quick Overview for E2F1 抗体 (pSer364) (ABIN214450)

抗原

See all E2F1 抗体
E2F1 (E2F Transcription Factor 1 (E2F1))

适用

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宿主

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克隆类型

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多克隆

标记

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This E2F1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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    pSer364

    特异性

    Amino acids 360-369 of Human E2F-1.

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide from human E2F1.

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ELISA (1:20000 - 1:100000), IHC, IHC-P (10 μg/mL), WB (1:250 - 1:2000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    E2F1 (E2F Transcription Factor 1 (E2F1))

    别名

    E2F1

    背景

    Name/Gene ID: E2F1

    Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

    基因ID

    1869

    UniProt

    Q01094

    途径

    p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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