SIRT1 抗体 (AA 207-256)
Quick Overview for SIRT1 抗体 (AA 207-256) (ABIN214144)
抗原
See all SIRT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 207-256
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品牌
- IHC-plus™
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特异性
- Human SIRT1
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预测反应
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS with 2 % sucrose
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- SIRT1 (Sirtuin 1 (SIRT1))
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别名
- SIRT1 / Sirtuin 1
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背景
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Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase
Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1 -
基因ID
- 23411
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NCBI登录号
- NP_036370
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UniProt
- Q96EB6
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途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
抗原
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