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SIRT1 抗体 (AA 207-256)

This anti-SIRT1 antibody is a 兔 多克隆 antibody detecting SIRT1 in WB, IHC 和 IHC (p). Suitable for 人, 小鼠, 大鼠, 犬, 兔, 猴, Cow, 豚鼠, 马, 斑马鱼, Bat 和 Pig.
产品编号 ABIN214144
发货至: 中国

Quick Overview for SIRT1 抗体 (AA 207-256) (ABIN214144)

抗原

See all SIRT1 抗体
SIRT1 (Sirtuin 1 (SIRT1))

适用

人, 小鼠, 大鼠, 犬, 兔, 猴, Cow, 豚鼠, 马, 斑马鱼, Bat, Pig

宿主

  • 148
  • 24
  • 2
  • 2
  • 1

克隆类型

  • 129
  • 48
多克隆

标记

  • 84
  • 15
  • 11
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
This SIRT1 antibody is un-conjugated

应用范围

  • 137
  • 53
  • 53
  • 50
  • 41
  • 39
  • 35
  • 30
  • 30
  • 16
  • 9
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 32
    • 16
    • 16
    • 12
    • 9
    • 7
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 207-256

    品牌

    IHC-plus™

    特异性

    Human SIRT1

    预测反应

    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (5 μg/mL), WB (0.5 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Distilled water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS with 2 % sucrose

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • 抗原

    SIRT1 (Sirtuin 1 (SIRT1))

    别名

    SIRT1 / Sirtuin 1

    背景

    Name/Gene ID: SIRT1
    Family: ADP-Ribosyltransferase

    Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1

    基因ID

    23411

    NCBI登录号

    NP_036370

    UniProt

    Q96EB6

    途径

    MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
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