AKT1 抗体 (pSer473)
Our Local Distributor
北京 101111
Quick Overview for AKT1 抗体 (pSer473) (ABIN213755)
抗原
See all AKT1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer473
-
特异性
- The C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide from human AKT1.
Type of Immunogen: Synthetic peptide
-
-
-
-
应用备注
-
Approved: ELISA (1:15000 - 1:60000), ICC, IF, IHC, IHC-P (5 μg/mL), WB (1:200 - 1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
-
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
-
别名
- AKT1 / AKT
-
背景
-
Name/Gene ID: AKT1
Subfamily: RAC/AKT
Family: Protein Kinase
Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA -
基因ID
- 207
-
UniProt
- P31749
-
途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
抗原
-