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ERBB3 抗体 (C-Term)

This anti-ERBB3 antibody is a 兔 多克隆 antibody detecting ERBB3 in WB 和 IP. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN213195
发货至: 中国

Quick Overview for ERBB3 抗体 (C-Term) (ABIN213195)

抗原

See all ERBB3 抗体
ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This ERBB3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)
  • 抗原表位

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    C-Term

    特异性

    Recognizes human HER3 at 180kD. Species cross-reactivity: mouse and rat. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum.

    纯化方法

    Protein A purified

    免疫原

    A GST-HER3 fusion protein corresponding to aa1283 to 1323 at the carboxy-terminus of human HER3.

    Type of Immunogen: Fusion protein

    亚型

    IgG
  • 应用备注

    Approved: IP, WB

    Usage: Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 5 μL. Use 25 μL of Protein A-agarose beads and 1 mL of lysate (lysate contains approximately 1 mg of total protein). Rotate mixture overnight at 4?C and then wash 3x with lysis buffer. Dissolve the resulting bead complex in 20-30 μL of 3xSDS-PAGE sample buffer and load ~15 μL per lane on an 8 % polyacrylamide gel. Positive control: Human prostate cell lines (LNCap) or human breast cell line (MDA-453).

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

    别名

    HER3 / ERBB3

    背景

    Name/Gene ID: ERBB3
    Subfamily: EGF Receptor
    Family: Protein Kinase

    Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3

    基因ID

    2065

    UniProt

    P21860

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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