EGFR 抗体
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北京 101111
Quick Overview for EGFR 抗体 (ABIN213194)
抗原
See all EGFR 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Species cross-reactivity: does not react with EGF receptors on mouse or rat cells.
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纯化方法
- Protein G purified
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免疫原
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Human A431 membrane proteins.
Type of Immunogen: Protein -
亚型
- IgG1
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应用备注
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Approved: ICC (10 μg/mL), IHC (10 μg/mL), IP, Neut, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Neutralization. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates the EGF receptor from 500 μg of non-stimulated A431 RIPA lysate. Immunocytochemistry: 10 μg/mL shows positive immunostaining for the EGF receptor in A431 cells fixed with 4 % para-formaldehyde. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile 40-50 % glycerol, ddH2O
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浓度
- Lot specific
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缓冲液
- Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride
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储存条件
- -20 °C
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储存方法
- Lyophilized and resuspended forms: store at -20°C.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
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Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1 -
基因ID
- 1956
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原
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