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EPH Receptor A2 抗体

This anti-EPH Receptor A2 antibody is a 小鼠 单克隆 antibody detecting EPH Receptor A2 in WB, IP, ICC 和 Func. Suitable for 人, 小鼠, 大鼠, 犬 和 Cow.
产品编号 ABIN211915
发货至: 中国

Quick Overview for EPH Receptor A2 抗体 (ABIN211915)

抗原

See all EPH Receptor A2 (EPHA2) 抗体
EPH Receptor A2 (EPHA2)

适用

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人, 小鼠, 大鼠, 犬, Cow

宿主

  • 113
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小鼠

克隆类型

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单克隆

标记

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This EPH Receptor A2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)
  • 特异性

    Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.

    纯化方法

    Protein G purified

    免疫原

    Native protein isolated by purification of phosphotyrosine-containing proteins.

    亚型

    IgG1
  • 应用备注

    Approved: Func, ICC, IP, WB

    Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    EPH Receptor A2 (EPHA2)

    别名

    EPHA2 / EPH Receptor A2

    背景

    Name/Gene ID: EPHA2
    Subfamily: Ephrin Receptor
    Family: Protein Kinase

    Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

    基因ID

    1969

    UniProt

    P29317

    途径

    RTK signaling
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