EPH Receptor A2 抗体
Quick Overview for EPH Receptor A2 抗体 (ABIN211915)
抗原
See all EPH Receptor A2 (EPHA2) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.
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纯化方法
- Protein G purified
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免疫原
- Native protein isolated by purification of phosphotyrosine-containing proteins.
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亚型
- IgG1
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应用备注
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Approved: Func, ICC, IP, WB
Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- EPH Receptor A2 (EPHA2)
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别名
- EPHA2 / EPH Receptor A2
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背景
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Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1 -
基因ID
- 1969
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UniProt
- P29317
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途径
- RTK signaling
抗原
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