CREB1 抗体 (pSer133)
Quick Overview for CREB1 抗体 (pSer133) (ABIN211133)
抗原
See all CREB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer133
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特异性
- Recognizes phospho-CREB (Ser133), Mr 43kD. Species cross-reactivity: Rat and mouse. Broad species cross-reactivity expected, based on sequence homology.
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纯化方法
- Protein G purified
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免疫原
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Proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB.
Type of Immunogen: Synthetic peptide -
亚型
- IgG1 kappa
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应用备注
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Approved: WB (1:1000)
Usage: Suitable for use in Western Blot. Western Blot: 1:1000 to 1:4000. Detects phospho-CREB (Ser133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Lysates from untreated (Lanes 1 and 3) and forskolin treated (Lanes 2 and 4) PC-12 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-CREB (Ser133) (1:4000 dilution, Lanes 1 and 2) or anti-CREB (1:2000 dilution, Lanes 3 and 4). Proteins were visualized using a goat antimouse (Lanes 1and 2) or goat antirabbit (Lanes 3 and 4) secondary antibody conjugated to HRP and a chemiluminescence detection system. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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别名
- CREB1 / CREB
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背景
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Name/Gene ID: CREB1
Family: Transcription factor
Synonyms: CREB1, CREB-1, Transactivator protein, CREB -
基因ID
- 1385
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UniProt
- P16220
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途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
抗原
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