CREB1 抗体 (AA 5-24)
Quick Overview for CREB1 抗体 (AA 5-24) (ABIN211132)
抗原
See all CREB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-24
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特异性
- Recognizes human CREB at 43kD. Species cross-reactivity: Broad species cross-reactivity expected due to conservation of immunogenic sequence.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%) Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%) Rat, Xenopus (90%).
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纯化方法
- Protein A purified
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免疫原
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KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%), Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%), Rat, Xenopus (90%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: IP, WB
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 mg/mL detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 5 mg (will immunoprecipitated CREB from 500 mg of HeLa nuclear extract). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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别名
- CREB1 / CREB
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背景
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Name/Gene ID: CREB1
Family: Transcription factor
Synonyms: CREB1, CREB-1, Transactivator protein, CREB -
基因ID
- 1385
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UniProt
- P16220
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途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
抗原
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