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CREB1 抗体 (AA 5-24)

This anti-CREB1 antibody is a 兔 多克隆 antibody detecting CREB1 in WB 和 IP. Suitable for 人, Pig, 犬, 马, 兔, 猴 和 Bat.
产品编号 ABIN211132
发货至: 中国

Quick Overview for CREB1 抗体 (AA 5-24) (ABIN211132)

抗原

See all CREB1 抗体
CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

适用

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人, Pig, 犬, 马, 兔, 猴, Bat

宿主

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克隆类型

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多克隆

标记

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This CREB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)
  • 抗原表位

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    AA 5-24

    特异性

    Recognizes human CREB at 43kD. Species cross-reactivity: Broad species cross-reactivity expected due to conservation of immunogenic sequence.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%) Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%) Rat, Xenopus (90%).

    纯化方法

    Protein A purified

    免疫原

    KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%), Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%), Rat, Xenopus (90%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    亚型

    IgG
  • 应用备注

    Approved: IP, WB

    Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 mg/mL detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 5 mg (will immunoprecipitated CREB from 500 mg of HeLa nuclear extract).

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

    别名

    CREB1 / CREB

    背景

    Name/Gene ID: CREB1
    Family: Transcription factor

    Synonyms: CREB1, CREB-1, Transactivator protein, CREB

    基因ID

    1385

    UniProt

    P16220

    途径

    TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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