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Nibrin 抗体 (AA 420-774)

This anti-Nibrin antibody is a 小鼠单克隆 antibody detecting Nibrin in WB 和 IP. Suitable for 人.

产品编号 ABIN210930

发货至: 中国

Quick Overview for Nibrin 抗体 (AA 420-774) (ABIN210930)

抗原

See all Nibrin (NBN) 抗体

Nibrin (NBN)

适用

94
61
35
2
1
1
1
1

人

宿主

小鼠

克隆类型

单克隆

标记

69
7
5
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1

This Nibrin antibody is un-conjugated

应用范围

104
42
31
29
27
26
22
16
15
11
6
4
2
2
1

Western Blotting (WB), Immunoprecipitation (IP)

Discover our top product NBN Primary Antibody

抗原表位
39

15

10

8

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 420-774

特异性

Recognizes human Nibrin. Recognizes Nibrin/Nbs1, Mr 95kD. A non-specific protein was also detected at Mr 33kD in some lysates, but not Jurkat cell lysate. Species cross-reactivity: Human, NOT mouse.

纯化方法

Protein G purified

免疫原

GST fusion protein corresponding to residues 420-774 of human Nibrin.

Type of Immunogen: Fusion protein

亚型

IgG
anti-Nibrin (NBN) (AA 467-615) antibody
NBN 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4C2 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 8E7 unconjugated

anti-Nibrin (NBN) (AA 461-754) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A9 unconjugated

anti-Nibrin (NBN) (AA 461-754) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A2 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 6E11 unconjugated

anti-Nibrin (NBN) (AA 183-460) antibody
NBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated

anti-Nibrin (NBN) (AA 435-754) antibody
NBN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Nibrin (NBN) (AA 645-754) antibody
NBN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E4 unconjugated

anti-Nibrin (NBN) antibody
KO Validated NBN 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Nibrin (NBN) (AA 714-745), (C-Term) antibody
NBN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Nibrin/Nbs1 in Jurkat, HeLa nuclear extract, HeLa whole cell, and A431+/-EGF lysates. Included Positive Antigen Control: Jurkat lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitates Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Nibrin/Nbs1 (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Nibrin/Nbs1 (95kD).

说明

Target Species of Antibody: Human

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

100 mM Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
抗原

Nibrin (NBN)

别名

NBN / NBS1 / Nibrin

背景

Name/Gene ID: NBN

Synonyms: NBN, ATV, AT-V2, Nibrin, NBS1, AT-V1, NBS, p95

基因ID

4683

UniProt

O60934

途径

DNA Damage Repair, Production of Molecular Mediator of Immune Response

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