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E2F1 抗体 (AA 1-89, AA 342-386)

This anti-E2F1 antibody is a 小鼠 单克隆 antibody detecting E2F1 in WB 和 IP. Suitable for 人 和 非洲爪蟾.
产品编号 ABIN210874
发货至: 中国

Quick Overview for E2F1 抗体 (AA 1-89, AA 342-386) (ABIN210874)

抗原

See all E2F1 抗体
E2F1 (E2F Transcription Factor 1 (E2F1))

适用

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人, 非洲爪蟾

宿主

  • 207
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小鼠

克隆类型

  • 194
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单克隆

标记

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This E2F1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)

克隆位点

KH20-KH95
  • 抗原表位

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    AA 1-89, AA 342-386

    特异性

    Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat, Elephant (94%) Opossum (89%) Chicken, Lizard (83%).

    纯化方法

    Protein G purified

    免疫原

    GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20, aa342-386 for clone KH95.

    Type of Immunogen: Fusion protein

    亚型

    IgG
  • 应用备注

    Approved: IP, WB

    Usage: Western Blot Analysis: 0.5-2 μg/mL of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 μg per lane. Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    E2F1 (E2F Transcription Factor 1 (E2F1))

    别名

    E2F1

    背景

    Name/Gene ID: E2F1

    Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3

    基因ID

    1869

    NCBI登录号

    NP_005216

    UniProt

    Q01094

    途径

    p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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