MMP14 抗体 (AA 319-333)
Quick Overview for MMP14 抗体 (AA 319-333) (ABIN209030)
抗原
See all MMP14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 319-333
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特异性
- Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).
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纯化方法
- Immunoaffinity purified
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免疫原
- Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).
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亚型
- IgG3 kappa
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anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 121-487) antibody
MMP14 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 112-246) antibodyMMP14 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 6A11D1 unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (C-Term) antibodyMMP14 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 470-499) antibodyMMP14 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 133CT15-10-5-1 unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 322-541) antibodyMMP14 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 145-174), (N-Term) antibodyMMP14 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB02027 unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (Internal Region) antibodyVerified MMP14 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 160-173) antibodyMMP14 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) antibodyMMP14 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14) (AA 112-321) antibodyMMP14 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)
Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.0, 2 % BSA (protease free).
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
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别名
- MMP14 / MMP-14
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背景
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Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease
Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1 -
基因ID
- 4323
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UniProt
- P50281
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途径
- Autophagy
抗原
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