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H2AFX 抗体 (pSer139)

This anti-H2AFX antibody is a 小鼠 单克隆 antibody detecting H2AFX in WB, IF, ICC 和 IP. Suitable for 人, Cow, 犬, 兔 和 猴.
产品编号 ABIN208587
发货至: 中国

Quick Overview for H2AFX 抗体 (pSer139) (ABIN208587)

抗原

See all H2AFX 抗体
H2AFX (H2A Histone Family, Member X (H2AFX))

适用

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人, Cow, 犬, 兔, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This H2AFX antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
  • 抗原表位

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    AA 134-142, pSer139

    特异性

    Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.

    纯化方法

    Protein G purified

    免疫原

    Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.

    Type of Immunogen: Synthetic peptide - KLH conjugated

    亚型

    IgG1
  • 应用备注

    Approved: ICC (2 μg/mL), IF, IP, WB

    Usage: Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 μg/mL detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    H2AFX (H2A Histone Family, Member X (H2AFX))

    别名

    H2AX

    背景

    Name/Gene ID: H2AFX

    Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

    基因ID

    3014

    UniProt

    P16104

    途径

    Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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