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EPH Receptor A2 抗体 (Extracellular Domain)

EPHA2 适用: 人, 小鼠, 犬, Cow WB, FACS, ICC 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN204192
发货至: 中国
  • 抗原 See all EPH Receptor A2 (EPHA2) 抗体
    EPH Receptor A2 (EPHA2)
    抗原表位
    • 19
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    • 9
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    Extracellular Domain
    适用
    • 170
    • 128
    • 70
    • 14
    • 5
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    • 1
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    人, 小鼠, 犬, Cow
    宿主
    • 181
    • 16
    • 4
    • 1
    • 1
    小鼠
    克隆类型
    • 178
    • 24
    • 1
    单克隆
    标记
    • 72
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    • 9
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    • 2
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    • 2
    This EPH Receptor A2 antibody is un-conjugated
    应用范围
    • 120
    • 70
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    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    特异性
    Recognizes human EphA2.
    纯化方法
    Protein G purified
    免疫原
    NS0-derived rhEphA2 extracellular domain.
    亚型
    IgG2a
    Top Product
    Discover our top product EPHA2 Primary Antibody
  • 应用备注
    Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute with 200 μL sterile PBS.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.4, 5 % trehalose
    注意事项
    Avoid frequent freeze thaws. Store undiluted.
    储存条件
    -20 °C,-80 °C
    储存方法
    Store at -20°C to -70°C. Stable for 1 year from date of receipt.
  • 抗原
    EPH Receptor A2 (EPHA2)
    别名
    EPHA2 / EPH Receptor A2 (EPHA2 产品)
    别名
    ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody
    背景
    Name/Gene ID: EPHA2
    Subfamily: Ephrin Receptor
    Family: Protein Kinase

    Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1
    基因ID
    1969
    UniProt
    P29317
    途径
    RTK signaling
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