EPH Receptor A2 抗体 (Extracellular Domain)
-
- 抗原 See all EPH Receptor A2 (EPHA2) 抗体
- EPH Receptor A2 (EPHA2)
-
抗原表位
- Extracellular Domain
-
适用
- 人, 小鼠, 犬, Cow
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This EPH Receptor A2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 特异性
- Recognizes human EphA2.
- 纯化方法
- Protein G purified
- 免疫原
- NS0-derived rhEphA2 extracellular domain.
- 亚型
- IgG2a
- Top Product
- Discover our top product EPHA2 Primary Antibody
-
-
- 应用备注
-
Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 200 μL sterile PBS.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
- 注意事项
- Avoid frequent freeze thaws. Store undiluted.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
-
- 抗原
- EPH Receptor A2 (EPHA2)
- 别名
- EPHA2 / EPH Receptor A2 (EPHA2 产品)
- 背景
-
Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1 - 基因ID
- 1969
- UniProt
- P29317
- 途径
- RTK signaling
-