EPH Receptor A2 抗体 (Extracellular Domain)
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Quick Overview for EPH Receptor A2 抗体 (Extracellular Domain) (ABIN204192)
抗原
See all EPH Receptor A2 (EPHA2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes human EphA2.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rhEphA2 extracellular domain.
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亚型
- IgG2a
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anti-EPH Receptor A2 (EPHA2) (AA 30-60), (N-Term) antibody
EPHA2 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB01579 unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 901-976) antibodyEPHA2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 204-326) antibodyEPHA2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E3 unconjugated
anti-EPH Receptor A2 (EPHA2) antibodyEPHA2 适用: 人 FACS, IF, cELISA 宿主: 小鼠 Monoclonal Kalpha-5H5 unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 30-60) antibodyEPHA2 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 333-347), (Extracellular) antibodyEPHA2 适用: 人 WB, IHC, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 847-976) antibodyEPHA2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A2 (EPHA2) (Tyr588), (Tyr596) antibodyEPHA2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-EPH Receptor A2 (EPHA2) (AA 559-976) antibodyEPHA2 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3D7 unconjugated
anti-EPH Receptor A2 (EPHA2) antibodyEPHA2 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1B3C7 unconjugated
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应用备注
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Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 200 μL sterile PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
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注意事项
- Avoid frequent freeze thaws. Store undiluted.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -70°C. Stable for 1 year from date of receipt.
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- EPH Receptor A2 (EPHA2)
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别名
- EPHA2 / EPH Receptor A2
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背景
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Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase
Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1 -
基因ID
- 1969
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UniProt
- P29317
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途径
- RTK signaling
抗原
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