His Tag 抗体 (iFluor 555)
Quick Overview for His Tag 抗体 (iFluor 555) (ABIN2017573)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- THE™
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序列
- HHHHHH
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产品特性
- THETM His Tag Antibody [iFluor 555], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
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纯化方法
- Protein A affinity column
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免疫原
- A synthetic peptide HHHHHH coupled to KLH
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亚型
- IgG1 kappa
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining:1-4 μg/mL
Other applications: user-optimized -
限制
- 仅限研究用
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注意事项
- Avoid repeated freezing and thawing cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
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有效期
- 12 months
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- His Tag
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物质类
- Tag
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背景
- The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.
抗原
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