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Sonic Hedgehog 抗体 (AA 199-437)

This 大鼠 单克隆 anti-Sonic Hedgehog antibody specifically detects Sonic Hedgehog in WB 和 ELISA. The antibody is reactive with 小鼠 samples.
产品编号 ABIN6757522
发货至: 中国
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Quick Overview for Sonic Hedgehog 抗体 (AA 199-437) (ABIN6757522)

抗原

See all Sonic Hedgehog (SHH) 抗体
Sonic Hedgehog (SHH)

适用

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小鼠

宿主

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大鼠

克隆类型

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  • 47
单克隆

标记

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This Sonic Hedgehog antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 抗原表位

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    AA 199-437

    特异性

    Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.

    纯化方法

    Protein G purified

    免疫原

    Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide, aa 199-437).

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 5 % trehalose.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    Sonic Hedgehog (SHH)

    别名

    Sonic Hedgehog / SHH

    背景

    Name/Gene ID: SHH
    Subfamily: Cysteine C46
    Family: Protease

    Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

    基因ID

    6469

    UniProt

    Q15465

    途径

    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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