Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody detects endogenous levels of total Histone H2A.X protein.
纯化方法
Affinity Chromatography using epitope-specific immunogen
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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背景
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.Synonyms: H2AFX, H2AX, H2a/x