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ERK1/2 抗体 (Thr202)

The 兔 多克隆 anti-ERK1/2 antibody (ABIN197485) specifically detects ERK1/2 in WB. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN197485
发货至: 中国
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Quick Overview for ERK1/2 抗体 (Thr202) (ABIN197485)

抗原

See all ERK1/2 (MAPK1/3) 抗体
ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This ERK1/2 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    Thr202

    特异性

    P44/42 MAP Kinase antibody detects endogenous levels of total p44/42 MAP Kinase protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-TP-E-Y).
  • 应用备注

    Western Blot: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C
  • 抗原

    ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

    别名

    ERK1 / ERK2

    背景

    Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK
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