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Ephrin B2 抗体 (Tyr316)

This 兔 多克隆 anti-Ephrin B2 antibody specifically detects Ephrin B2 in WB. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN197416
发货至: 中国
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Quick Overview for Ephrin B2 抗体 (Tyr316) (ABIN197416)

抗原

See all Ephrin B2 (EFNB2) 抗体
Ephrin B2 (EFNB2)

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This Ephrin B2 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    Tyr316

    特异性

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Ephrin-B2 antibody AP02707PU detects endogenous levels of total Ephrin-B2 protein.

    纯化方法

    Immunoaffinity chromatography.

    免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B2 around the phosphorylation site of tyrosine 316 (P-V-Yp-I-V).
  • 应用备注

    Suitable for use in in Western blot (1: 500-1: 1000).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at -20 °C.
  • 抗原

    Ephrin B2 (EFNB2)

    别名

    Ephrin-B2

    背景

    Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5

    基因ID

    1948

    NCBI登录号

    NP_004084

    UniProt

    P52799

    途径

    RTK signaling, Regulation of Muscle Cell Differentiation
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