CDC25A 抗体 (Ser75)
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北京 101111
Quick Overview for CDC25A 抗体 (Ser75) (ABIN197370)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser75
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特异性
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. cdc25A antibody AP02684PU detects endogenous levels of total cdc25A protein.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-Sp-S-E).
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应用备注
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Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- CDC25A
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背景
- Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
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基因ID
- 993
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NCBI登录号
- NP_001780
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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