PRKDC 抗体 (pThr2609)
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北京 101111
Quick Overview for PRKDC 抗体 (pThr2609) (ABIN196898)
抗原
See all PRKDC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr2609
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特异性
- DNA PKcs (phospho-Thr2609) antibody detects endogenous levels of DNA PKcs only when phosphorylated at threonine 2609.
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纯化方法
- Immunoaffinity chromatography using epitope-specific phosphopeptide.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human DNA PKcs around the phosphorylation site of threonine 2609 (V-E-TP-Q-A).
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应用备注
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Suitable for use in Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
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别名
- DNA-PKcs / PRKDC / XRCC7
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背景
- DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
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基因ID
- 5591
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NCBI登录号
- NP_001075109
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UniProt
- P78527
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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