FZD1 抗体
Quick Overview for FZD1 抗体 (ABIN1967975)
抗原
See all FZD1 (Fzd1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes mouse Frizzled-1. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7, -8 or rhMFRP. By Flow Cytometry, detects Frizzled-1 on differentiated embryoid bodies derived from the mouse embryonic cell line D3. Also recognizes Frizzled-1 on human HEK 293 cells by Flow Cytometry.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rmFrizzled-1 CRD.
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亚型
- IgG2a
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应用备注
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Approved: ELISA, Flo, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry and ELISA. Western Blot: 1-2 μg/mL. With a colorimetric detection system, the detection limit is ~5 ng/lane and ~25 ng/lane under non-reducing and reducing conditions, respectively. Flow Cytometry: 0.1 mg/mL, Add 10 μL to label1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Visualized by using a secondary antibody (goat anti-rat IgG (FITC)). ELISA (Direct): Can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~10 ng/well. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Reconstitute with 200 μL sterile PBS.
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浓度
- Lot specific
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缓冲液
- PBS, 5 % Trehalose.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
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别名
- FZD1 / Frizzled 1
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背景
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Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1 -
基因ID
- 8321
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途径
- WNT signaling, Asymmetric Protein Localization
抗原
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