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FZD1 抗体

The 大鼠 单克隆 anti-FZD1 antibody is suitable to detect FZD1 in samples from 人 和 小鼠. It has been validated for WB, ELISA 和 FACS.
产品编号 ABIN1967975
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for FZD1 抗体 (ABIN1967975)

抗原

See all FZD1 (Fzd1) 抗体
FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

适用

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人, 小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This FZD1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS)
  • 特异性

    Recognizes mouse Frizzled-1. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7, -8 or rhMFRP. By Flow Cytometry, detects Frizzled-1 on differentiated embryoid bodies derived from the mouse embryonic cell line D3. Also recognizes Frizzled-1 on human HEK 293 cells by Flow Cytometry.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rmFrizzled-1 CRD.

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA, Flo, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Flow Cytometry and ELISA. Western Blot: 1-2 μg/mL. With a colorimetric detection system, the detection limit is ~5 ng/lane and ~25 ng/lane under non-reducing and reducing conditions, respectively. Flow Cytometry: 0.1 mg/mL, Add 10 μL to label1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Visualized by using a secondary antibody (goat anti-rat IgG (FITC)). ELISA (Direct): Can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~10 ng/well.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Reconstitute with 200 μL sterile PBS.

    浓度

    Lot specific

    缓冲液

    PBS, 5 % Trehalose.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

    别名

    FZD1 / Frizzled 1

    背景

    Name/Gene ID: FZD1
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

    基因ID

    8321

    途径

    WNT signaling, Asymmetric Protein Localization
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