ASS1 抗体 (Internal Region)
(1 reference)Quick Overview for ASS1 抗体 (Internal Region) (ABIN190834)
抗原
See all ASS1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Argininosuccinate synthetase 1
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序列
- ENPKNQAPPG LYTKTQD
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特异性
- The variants represent identical protein (NP_000041.2 and NP_446464.1).
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- C-ENPKNQAPPGLYTKTQD
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亚型
- IgG
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anti-Argininosuccinate Synthase 1 (ASS1) (AA 1-412) antibody
ASS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) antibodyASS1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) antibodyASS1 适用: 人, 小鼠, 猴 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 2C10 unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) (AA 192-221) antibodyASS1 适用: 人, 小鼠, 大鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB20920 unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) antibodyASS1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) (AA 192-221) antibodyASS1 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) (AA 281-310) antibodyASS1 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) antibodyASS1 适用: 人, 小鼠, 大鼠, 猴 WB, FACS, IF, EIA 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) (AA 1-412) antibodyASS1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Argininosuccinate Synthase 1 (ASS1) (AA 281-310), (C-Term) antibodyASS1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB32051 unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
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: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).
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- ASS1 (Argininosuccinate Synthase 1 (ASS1))
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别名
- ASS1
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背景
- Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
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基因ID
- 445, 11898, 25698
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NCBI登录号
- NP_000041, NP_446464
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途径
- Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
抗原
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