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E2F1 抗体 (AA 133-322)

This 兔 多克隆 antibody specifically detects E2F1 in WB 和 IF. It exhibits reactivity toward 人.
产品编号 ABIN1886014
发货至: 中国
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Quick Overview for E2F1 抗体 (AA 133-322) (ABIN1886014)

抗原

See all E2F1 抗体
E2F1 (E2F Transcription Factor 1 (E2F1))

适用

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宿主

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克隆类型

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多克隆

标记

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This E2F1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    AA 133-322

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 133 and 322 (Q01094) of E2F1
  • 应用备注

    Suggested dilutions:
    Western blotting: 1.500-1.3000
    Immunofluorescence: 1.100-1.200

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    Biohazard Informations: This product contains thimerosal which is hazardous.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • 抗原

    E2F1 (E2F Transcription Factor 1 (E2F1))

    别名

    E2F1

    背景

    The protein encoded by this gene is a member of the E2F family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.The E2F proteins contain several evolutionally conserved domains found in most members of the family.These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain.This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner.It can mediate both cell proliferation and p53-dependent/independent apoptosis.[provided by RefSeq]

    分子量

    47 kDa

    基因ID

    1869

    NCBI登录号

    NM_005225, NP_005216

    途径

    p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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