Keratin Acidic (AE1) 抗体
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Quick Overview for Keratin Acidic (AE1) 抗体 (ABIN187689)
抗原
See all Keratin Acidic (AE1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- AE-1 detects in immunoblots distinct bands of 40 kDa, 45 kDa, 46 kDa, 48 kDa, 50 kDa, (50' kDa), 51 kDa, 55 kDa, and 56.5 kDa with all Ip's lower than 5.7. These MW's are believed to correspond with keratin 19, 18, 17, 16, 15, 14, 13, 12 and 10, respectively. Species Reactivity: Human, bovine, chicken, mouse, rabbit, and rat. Others not tested.
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亚型
- IgG1
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anti-Keratin Acidic (AE1) antibody
AE1 适用: 人 IHC, WB 宿主: 大鼠 Monoclonal OTI1D10 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鼠, 小鸡, Cow, 兔, 猴, Pig IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyCarrier-free AE1 适用: 人, 大鼠 IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin Acidic (AE1) antibodyCarrier-free AE1 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
anti-Keratin Acidic (AE1) antibodyCarrier-free AE1 适用: 人 IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
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应用备注
- Immunohistochemistry: (Formalin-fixed / Paraffin-embedded sections) Use at 1:150-1:200 for 30 min. at 37 °C. Higher dilutions can be used if sections are incubated at 4 °C overnight. Western blot: Use at 1:100. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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缓冲液
- 250 µg purified IgG at 1 mg/ml with 0.1% sodium azide.
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储存条件
- 4 °C
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- Keratin Acidic (AE1)
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别名
- Cytokeratin (Epithelial type I)
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背景
- This antibody recognizes most of the acidic (type I) keratins. Each epithelium contains at least one acidic keratin. AE-1 is broadly reactive with almost all epithelial and their neoplasms. The antibody exhibits heterogenous staining within a given epithelium, the detailed staining pattern varies depending on the differentiated and/or growth state of the individual cells. This antibody together with MC-976 (clone AE-3) has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelial development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma.
抗原
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