Caspase 2 抗体
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北京 101111
Quick Overview for Caspase 2 抗体 (ABIN187544)
抗原
See all Caspase 2 (CASP2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51 kDa (procaspase-2), ~32 kDa and ~18 kDa (cleavage products) by Western blot. Species Reactivity: Human, mouse, rat, monkey and dog. Others not tested.
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免疫原
- p19 fragment of recombinant human Caspase-2.
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亚型
- IgG2a
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应用备注
- Immunoprecipitation Western blot: Use at 2 µg/mL. The optimal dilution for a specific application should be determined by the researcher. Figure (left column): Detection of procaspase-2 (p51), an intermediate cleavage product of caspase-2 (p32) and activated caspase-2 (p19) in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor withdrawal induced apoptosis. Activation of caspase-2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK (AB-001). Figures (above): Western blot analysis of cell lysates from human and mouse cell lines with clone 11B4 (MC-498). Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous procaspase-2. Hsp-70 was used as an equal loading control, and -/- caspase-2 cells were used as a negative control.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 µL of protein G affinity-purified monoclonal antibody at 1 mg/mL in PBS containing 0.02% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))
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别名
- Caspase-2
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway
抗原
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