FASL 抗体
Quick Overview for FASL 抗体 (ABIN187289)
抗原
See all FASL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Species Reactivity: Mouse. Others not tested.
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纯度
- > 95 % by SDS-PAGE.
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免疫原
- Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).
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亚型
- IgG2a
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anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-281) antibody
FASL 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-200) antibodyFASL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 102-280) antibodyFASL 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-278) antibodyFASL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 103-281) antibodyFASL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 82-145) antibodyFASL 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) antibodyFASL 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2G9-G8 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal NOK-1 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal NOK-1 unconjugated
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应用备注
- Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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别名
- CD178 / Fas Ligand
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背景
- Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.
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途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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