FASL 抗体
-
- 抗原 See all FASL 抗体
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This FASL antibody is un-conjugated
-
应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced apoptosis of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and some tumor cells. Species Reactivity: Mouse. Others not tested.
- 免疫原
- Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein
- 克隆位点
- A11
- 亚型
- IgM
- Top Product
- Discover our top product FASL Primary Antibody
-
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-281) antibody
FASL 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-200) antibodyFASL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 102-280) antibodyFASL 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-278) antibodyFASL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 103-281) antibodyFASL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 82-145) antibodyFASL 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) antibodyFASL 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2G9-G8 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal NOK-1 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal NOK-1 unconjugated
-
- 应用备注
- Flow cytometry Immunohistochemistry: Excellent for this application. Frozen Sections- Use 20 µg/mL, incubate 1 hr at room temp. Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at RT with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Paraffin-Embedded Sections- Dewax in xylene (2 x 10 min), EtOH 100% (2 x 10 min), block endogenous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H2O, unmask epitopes by heating slides in citrate buffer (0.01 M, pH 6.0) 2 x 5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). The optimal dilution for a specific application should be determined by the researcher.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 µL of 1 mg/mL monoclonal antibody in PBS with 0.02% sodium azide. Purity 95% by SDS-PAGE.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- 别名
- CD178 / Fas Ligand (FASL 产品)
- 别名
- ALPS1B antibody, APT1LG1 antibody, APTL antibody, CD178 antibody, CD95-L antibody, CD95L antibody, FASL antibody, TNFSF6 antibody, fasl antibody, Fas-L antibody, Faslg antibody, Tnfsf6 antibody, gld antibody, Apt1Lg1 antibody, Fasl antibody, FASLG antibody, zgc:162027 antibody, Fas ligand antibody, Fas ligand L homeolog antibody, Fas ligand (TNF superfamily, member 6) antibody, FASLG antibody, faslg.L antibody, Fasl antibody, Faslg antibody, faslg antibody
- 途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
-
