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Patched 1 抗体 (Internal Region)

Cited in 1 publication. This 山羊 多克隆 anti-Patched 1 antibody specifically detects Patched 1 in WB, ELISA 和 IF. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN185667
发货至: 中国
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Quick Overview for Patched 1 抗体 (Internal Region) (ABIN185667)

抗原

See all Patched 1 (PTCH1) 抗体
Patched 1 (PTCH1)

适用

  • 44
  • 28
  • 10
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
人, 小鼠

宿主

  • 39
  • 7
  • 3
  • 3
山羊

克隆类型

  • 43
  • 9
多克隆

标记

  • 40
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Patched 1 antibody is un-conjugated

应用范围

  • 34
  • 23
  • 19
  • 11
  • 6
  • 6
  • 3
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    PTCH (Internal)

    序列

    HPESRHHPPS NPRQQ

    特异性

    This antibody is expected to recognise all four reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1). Reported variants represent identical protein (NP_001077075.1, NP_001077076.1, NP_001077073.1, NP_001077074.1).

    交叉反应

    Cow, 犬, 人, Pig

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

  • 抗原

    Patched 1 (PTCH1)

    别名

    PTCH1

    背景

    PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog

    基因ID

    5727

    NCBI登录号

    NP_000255, NP_001077072, NP_001077071, NP_001077075

    途径

    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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